Fragment Specificity And Antibody Selection

Fragment Specificity And Antibody Selection


Immunoassays such as Western blotting and ELISA often rely on a two-stage technique. Initially, a primary antibody (1o Ab) is used to bind to the protein of interest. A secondary antibody (2o Ab), labelled with a detection tag such as a fluorescent dye or colourmetric enzyme, is then applied. This binds to a site on the primary Ab, allowing indirect detection of the antigen.Single stage assays have been developed, in which

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